Features and Benefits

Compatible with high-throughput handling systems.

General description

Creatine Kinase (CK), also known as phosphocreatine kinase, is an enzyme that catalyzes the transfer of one phosphate group from ATP to creatine generating phosphocreatine, an important energy reservoir in muscle and brain tissue. CK is a dimeric protein made up of B (brain) and M (muscle) subunits. Three isoenzymes, CK-MM, CK-MB, and CK-BB, have been observed. CK levels are elevated in various pathological conditions including myocardial infarction, rhabdomyolysis, muscular dystrophy, and renal failure.

Principle

Creatine Kinase activity is determined by a coupled enzyme reaction resulting in the production of NADPH, measured at 340 nm, proportionate to the CK activity present in the sample. In this reaction, phosphocreatine and ADP are converted to creatine and ATP. The generated ATP is used by hexokinase to phosphorylate glucose resulting in glucose-6-phosphate, which is oxidized by NADP in the presence of glucose-6-phosphate dehydrogenase to produce NADPH and 6-phospho-D-gluconate. One unit of CK is the amount of enzyme that will transfer 1.0 µmole of phosphate from phosphocreatine to ADP per minute at pH 6.0. This kit has a linear range of 30–1,800 units/L CK activity.

Suitability

Suitable for the detection of Creatine Kinase activity in a variety of samples such as blood, serum, and plasma. This kit can be used to study the effects of various compounds on Creatine Kinase activity.